EST isozymes are one of the frequently used biochemical markers in genetic analysis of fungi, and the staining is an important
process in electrophoresis analysis of studying Est. The effects were compared among different stain recipes for Est of 3
kinds of fungi-Lentinus
edodes,
Pleurotus sapidus, Phellinus igriarius and 2 kinds of
plants-Populus sp and
Brassica chinensis. Of the four kinds of Est staining recipes tested, the recipe α-acetic acid-naphther showed the best effect. and followed
by β-aceticacid-naphther, semicontent α-aceticacid-naphther and α+β-aceticacid-nathpher.
Key words Fungi - Esterase isozyme - Staining methods