Monascus pilosus NBRC4520 was selected for functional fermented food inoculation for its high lovastatin and low citrinin production with
a deep-red color. For koji (mold rice) with high lovastatin production, separation of the growth phase and lovastatin production
phase by shifting the temperature from 30 to 23 °C increased lovastatin production by nearly 20 times compared to temperature-constant
cultivation. In addition, citrinin was not produced even in the lovastatin production phase, although the pigment was increased.
With temperature-shift cultivation, 225 μg lovastatin/g dry koji was produced in 14 days without citrinin.
Keywords Monascus - Lovastatin - Temperature-shift cultivation - Pigment - Citrinin