1-9
Expanded bed adsorption: elution in expanded bed mode
Rolf Hjorth
3
Preface
Preface
11-31
Monitoring, modeling, and control strategies for expanded-bed adsorption processes
Travis V. Thelen and W. Fred Ramirez
33-41
Factors affecting dispersion in expanded bed chromatography
Shuichi Yamamoto, Nagomu Akazaki, Oliver Kaltenbrunner and Peter Watler
43-51
Physical and biochemical characterization of a simple intermediate between fluidized and expanded bed contactors
John Chi-Wei Lan, Grant E Hamilton and Andrew Lyddiatt
53-67
On-line monitoring of the purification of GST-(His)6 from an unclarified Escherichia coli homogenate within an immobilised metal affinity expanded bed
R.H. Clemmitt, L.J. Bruce and H.A. Chase
69-75
The effect of column verticality on separation efficiency in expanded bed adsorption
L.J. Bruce, S. Ghose and H.A. Chase
77-83
Evaluation of the effect of in-bed sampling on expanded bed adsorption
L.J. Bruce and H.A. Chase
85-91
EBA columns with a distribution system based on local stirring
Elias Zafirakos and Allan Lihme
93-97
Simplified and more robust EBA processes by elution in expanded bed mode
Allan Lihme, Elias Zafirakos, Marie Hansen and Morten Olander
99-109
Cell/adsorbent interactions in expanded bed adsorption of proteins
Jan Feuser, Joachim Walter, Maria-Regina Kula and Jörg Thömmes
111-114
Validation issues related to expanded bed technology
Gail Sofer
115-120
Highly dense beaded sorbents suitable for fluidized bed applications
N. Voute and E. Boschetti
121-129
Characterization of very dense mineral oxide–gel composites for fluidized-bed adsorption of biomolecules
Nicolas Voute, Damien Bataille, Pierre Girot and Egisto Boschetti
131-138
Expanded bed adsorption on supermacroporous cross-linked cellulose matrix
Anita Pai, Shyamal Gondkar, Sumati Sundaram and Arvind Lali
139-144
Characterisation of STREAMLINETM Phenyl
Johan Färenmark, Jan Gustavsson, Inger Lagerlund and Lena Sandberg
145-151
Use of Streamline chelating for capture and purification of poly-His-tagged recombinant proteins
Santosh Noronha, Jeanne Kaufman and Joseph Shiloach
153-158
A simplified purification procedure of α-lactalbumin from milk using Ca2+-dependent adsorption in hydrophobic expanded bed chromatography
Wim Noppe, Petra Haezebrouck, Ignace Hanssens and Marcel De Cuyper
159-168
Expanded bed adsorption as a unique unit operation for the isolation of bacteriocins from fermentation media
Raf Callewaert and Luc De Vuyst
169-188
Pilot scale recovery of monoclonal antibodies by expanded bed ion exchange adsorption
Nicole Ameskamp, Christoph Priesner, Jürgen Lehmann and Dirk Lütkemeyer
189-193
Capture of human Fab fragments by expanded bed adsorption with a mixed mode adsorbent
Marie Bendix Hansen, Allan Lihme, Mari Spitali and David King
195-200
Purification of Recombinant Human Serum Albumin Efficient purification using STREAMLINE
Akinori Sumi, Kouzou Okuyama, Kaoru Kobayashi, Wataru Ohtani and Takao Ohmura, et al.
201-207
Direct coupling of expanded bed adsorption with a downstream purification step
Joanne T. Beck, Brian Williamson and Barbara Tipton
209-217
Production of plasmid DNA for human gene therapy using modified alkaline cell lysis and expanded bed anion exchange chromatography
D.L. Varley, A.G. Hitchcock, A.M.E. Weiss, W.A. Horler and R. Cowell, et al.
219-228
Development and scale up of a capture step (expanded bed chromatography) for a fusion protein expressed intracellularly in Escherichia coli
Michael Brobjer
229-235
On-line monitoring of glucose and/or lactate in a fermentation process using an expanded micro-bed flow injection analyser
M.P. Nandakumar, Arvind M. Lali and B. Mattiasson
237-245
Binding assays in heterogeneous media using a flow injection system with an expanded micro-bed adsorption column
Bo Mattiasson and M. P. Nandakumar
247-254
Use of a micro-expanded bed containing immobilised lysozyme for cell disruption in flow injection analysis
M. P. Nandakumar, Anita Tocaj and B. Mattiasson
255-267
Flow injection analysis of intracellular β-galactosidase in Escherichia coli cultivations, using an on-line system including cell disruption, debris separation and immunochemical quantification
Anita Tocaj, M.P. Nandakumar, O. Holst and B. Mattiasson