A potential maximal clique of a graph is a vertex set that induces a maximal clique in some minimal triangulation of that graph. It is known that if these objects can be listed in polynomial time for a class of graphs, the treewidth and the minimum fill-in are polynomially tractable for these graphs. We show here that the potential maximal cliques of a graph can be generated in polynomial time in the number of minimal separators of the graph. Thus, the treewidth and the minimum fill-in are polynomially tractable for all graphs with polynomial number of minimal separators.