A method has been developed to detect inhomogeneity of apparently homogeneous peaks of very similar analytes. The method utilizes
the rapid scan feature of state-of-the-art spectrometers/detectors that allow the recording of up to 30 spectra in a single
chromatographic peak. Sensitivity and selectivity are enhanced by chiroptical/optical detection. Thus, identification of “front”
and “rear” components of the peak can be carried out. The method is exemplified by mixtures of codeine, hydrocodone and oxycodone
as analytes.
Key Words Column liquid chromatography - Circular dichroism detection - Multiscan detection - Peak purity
Presented at: Balaton Symposium on High-Performance Separation Methods, Siófok, Hungary, September 3–5, 1997