The orientation of oriented strand board (OSB) mats has been practically measured on a commercial factory production line to demonstrate the practical capabilities of the filtered image analysis (FIA) technique. Samples have been cut from OSB panels at a range of angles to the panel axis and these samples have been tested in bending. The factory data and the experimental data have been compared in order to investigate the relationship between the practical condition of fiber orientation in the factory and the bending properties as a function of orientation. The following conclusions can be drawn. Fiber orientation in the production line is good and stable irrespective of position across the width of the production line, time of day and changing line speed. The average value for the orientation angle of the forming mat on the production line is approximately 25°. The general shape of the fiber orientation distribution is similar to a normal distribution, however, at the centre of the fiber mat the sharpness of the distribution is greater than a normal distribution. The average orientations of fibers in commercial board lie at 25° and 60° to the longitudinal and perpendicular directions, respectively. The results suggest that there is potential to improve the mean fiber orientation angle of commercial OSB to improve longitudinal values of MOR and MOE, especially where perpendicular properties are not critical.