Addition of 40 g NaCl l
–1 to a chemically defined medium containing 140 g glucose l
–1 in shake-flask culture improved glycerol production by
Candida krusei from 16.5 g l
–1 to 47.7 g l
–1. With 40 g NaCl l
–1 at a dilution rate of 0.065 h
–1, glycerol concentration, glycerol yield (based on glucose consumed), and productivity in a four-stage cascade bioreactor were higher by 240%, 27% and 28%, respectively, than in a single-stage continuous culture system.
Candida krusei
- glycerol - multistage cascade bioreactor - NaCl - osmoregulator