The effects of cytokinins on somatic embryogenesis in pepper were studied using a Murashige and Skoog-based medium supplemented
with 2 mg/L 2,4-dichlorophenoxyacetic acid and 10% sucrose. Compared to the medium without cytokinins, N
6-(Δ
2-isopentenyl)-adenine and kinetin had no significant effect, whereas benzylaminopurine and zeatin significantly reduced somatic
embryogenesis. Coconut water (10%) either had no significant effect or it reduced embryogenesis as well. Induction of somatic
embryogenesis was also dependent on genotype. Appearance of somatic embryos continued after removal of the embryos that developed
first, especially on cultivars with a lower initial induction rate. Although somatic embryos germinated, the apical meristem
did not elongate.
Keywords Apical meristem - Cytokinin - Coconut water - Embryogenesis - Hormone - Pepper - Somatic embryo