Purpose: We evaluated the ability of optic disc parameters and nerve fiber analysis with the Glaucoma-scope to distinguish glaucoma from normal or hypertensive eyes. Methods: Glaucoma-scope examination was performed on 68 eyes of 44 patients split into following groups based on Humphrey 24-2 visual field and clinical criteria of glaucoma: normal eyes (n = 17), ocular hypertension (n = 19), early glaucoma (n = 17), moderately advanced glaucoma (n = 15). The contour of the juxta papillary nerve fiber layers was measured in the four groups. We also evaluated a global index as the mean disc depth corrected for the disc size by dividing by the disc area (MP/D). Results: We found a statistical difference (p < 0.001) between the four groups with the global index MP/D: normal eyes MP/D = – 17.42, hypertensive eyes MP/D = – 26.34, early glaucoma MP/D = – 82.33, moderately advanced glaucoma MP/D = – 99.69. We also found a statistical difference between the first two groups (normal visual field) compared to the two glaucoma groups for the mean fiber layer height in the superior and the inferior quadrant. Conclusion: Nerve fiber analysis and optic nerve head index calculated with the Glaucoma-scope could prove to be an interesting tool for glaucoma diagnosis and follow-up.