This study used self-report, facial EMG, galvanic skin response, and eye tracking to assess users’ participants’ responses to two charity web sites. For each site, page content and colour of presentation (colour versus black & white) were manipulated. Results support the utility and diversity of these measures. Each provided information about users’ responses to web page designs, with a good deal of variation in sensitivity to experimental manipulations. In combination the data from these measures allowed further inferences to be drawn, supporting a multi-dimensional view of user experience and the need for multi-method approaches to evaluation.