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Precipitation of Silver-Thiosulfate Complex and Immobilization of Silver by Cupriavidus metallidurans CH34

Marie-Laure Ledrich1, Sébastien Stemmler1, Philippe Laval-GillyContact Information, Laurent Foucaud1 and Jaïro FallaContact Information

(1)  IUT Thionville-Yutz, Laboratoire d’Immunologie – Microbiologie (ESE-CNRS, UMR 7146), 1 Impasse Alfred Kastler, 57970 Yutz, France

Received: 28 July 2005  Accepted: 7 October 2005  

Abstract  Cupriavidus metallidurans CH34 is a facultative chemolithotrophic bacterium that possesses two megaplasmids (pMOL28 and pMOL30) that confer resistance to eleven metals. The ability of Cupriavidus metallidurans CH34 to resist silver is described here. Electronic microscopy, energy-dispersive X-ray (EDX) and X-ray diffractometry (DRX) observations revealed that C. metallidurans CH34 strongly associated silver with the outer membrane, under chloride chemical form. Using derivate strains of C. metallidurans CH34, which carried only one or no megaplasmid, we show that this resistance seems to be carried by pMOL30.

Key words  Cupriavidus metallidurans CH34 - plasmid pMOL30 - silver


Contact Information Philippe Laval-Gilly (Corresponding author)
Email: plg@iut.univ-metz.fr
Phone: +33382820629
Fax: +33382820613

Contact Information Jaïro Falla (Corresponding author)
Email: jfalla@iut.univ-metz.fr
Phone: +33382820629
Fax: +33382820613
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