A model for DNA implementation of Royal Road evolutionary computations is presented. An encoding for a Royal Road problem is presented. Experimental results utilizing 2-d denaturing gradient gel electrophoresis (2-d DGGE) and polyacrylamide gel electrophoresis (PAGE) for separation by fitness in this sample Royal Road problem are shown. Suggestions for possible use of the MutS and MutY proteins as tools for separation by fitness are given. Plans for future experiments and implementation are discussed.