This paper reports three experimental achievements in our computation model based on ‘whiplash’ reactions. We first show that a single-stranded DNA (ssDNA) can serve as an independent machine by using a solid support technique. Second, we show how to append an arbitrary sequence, e.g. a transition state or a PCR primer, to the 3′- end of a molecular machine, thus realizing its I/O interface. Finally we demonstrate the successive state transitions for several steps on solid phase with I/O.