Micropropagation of
Scabiosa caucasica cv. Caucasica Blue was achieved by culturing, separating axillary and adventitious shoots, or node sectioning on Murashige
and Skoog (MS) medium supplemented with benzyladenine (BA). The highest frequency of adventitious shoots regenerated from
nodal or internodal explants and leaf blade (with or without petiole) appeared to occur on MS medium with 4.4 and 18 μ
M BA, respectively. Addition of 0.19 or 1.9 μ
M α-naphthaleneacetic acid to the BA-containing medium promoted callus formation and reduced shoot organogenesis. During micropropagation,
shoot nodal explants derived from
in vitro shoots cultured on MS medium supplemented with 4.4 μ
M BA yielded 8.9 shoots per explant within 40 d after culture initiation.
Key words micropropagation -
Scabiosa caucasica
- shoot regeneration - tissue culture