We report 11 novel microsatellite primer pairs for the wild tea,
Camellia sinensis (L.) O. Kuntze forma
formosensis Kitamura
. These simple sequence repeat markers were tested in 24 samples collected from wild tea populations, and in cultivars and
C. japonica. The number of alleles ranged from 4 to18. The expected (
H
E) and observed (
H
O) heterozygosity were 0.687–0.946 and 0.042–0.792, respectively. All loci were significantly deviated from Hardy-Weinberg
expectations due to the heterozygote deficiency, indicating a dramatic loss of genetic polymorphisms in the rare species.
Significant LD was discovered in most loci. These primers may provide a tool for understanding demography and population structure
in wild tea.
Keywords
Camellia
sinensis
- Heterozygosity - Microsatellite fingerprinting - PIMA - RAPD-PCR enrichment