i-xiii
Front matter
I / Sample Preparation Basics
1-1
Front matter
3-22
Mechanical/Physical Methods of Cell Disruption and Tissue Homogenization
Stanley Goldberg
23-34
Bacteria and Yeast Cell Disruption Using Lytic Enzymes
Oriana Salazar
35-42
Sample Solublization Buffers for Two-Dimensional Electrophoresis
Walter Weiss and Angelika Görg
43-49
Quantitation of Protein in Samples Prepared for 2-D Electrophoresis
Tom Berkelman
51-62
Removal of Interfering Substances in Samples Prepared for Two-Dimensional (2-D) Electrophoresis
Tom Berkelman
63-69
Protein Concentration by Hydrophilic Interaction Chromatography Combined with Solid Phase Extraction
Ulrich Schneider
II / Protein Labeling Techniques
71-71
Front matter
73-85
Difference Gel Electrophoresis Based on Lys/Cys Tagging
Reiner Westermeier and Burghardt Scheibe
87-99
Isotope-Coded Two-Dimensional Maps: Tagging with Deuterated Acrylamide and 2-Vinylpyridine
Pier Giorgio Righetti, Roberto Sebastiano and Attilio Citterio
101-111
Stable Isotope Labeling by Amino Acids in Cell Culture (SILAC)
s Gruhler and Irina Kratchmarova
113-123
ICPL—Isotope-Coded Protein Label
Josef Kellermann
125-135
Radiolabeling for Two-Dimensional Gel Analysis
Hélian Boucherie, Aurélie Massoni and Christelle Monribot-Espagne
III / Fractionation of Proteins by Chemical Reagents and Chromatography
137-137
Front matter
139-146
Sequential Extraction of Proteins by Chemical Reagents
Stuart J. Cordwell
147-156
Reducing Sample Complexity by RP-HPLC: Beyond the Tip of the Protein Expression Iceberg
Gert Van den Bergh and Lutgarde Arckens
157-166
Enriching Basic and Acidic Rat Brain Proteins with Ion
Exchange Mini Spin Columns Before Two-Dimensional
Gel Electrophoresis
Ning Liu and Aran Paulus
167-185
Reducing the Complexity of the Escherichia coli Proteome by Chromatography on Reactive Dye Columns
Phillip Cash and Ian R. Booth
187-203
Reducing Sample Complexity in Proteomics
by Chromatofocusing with Simple Buffer Mixtures
Hong Shen, Xiang Li, Charles J Bieberich and Douglas D Frey
205-212
Fractionation of Proteins by Immobilized Metal Affinity Chromatography
Xuesong Sun, Jen-Fu Chiu and Qing-Yu He
213-221
Fractionation of Proteins by Heparin Chromatography
Sheng Xiong, Ling Zhang and Qing-Yu He
IV / Fractionation of Proteins by Electrophoresis Methods
223-223
Front matter
225-239
Fractionation of Complex Protein Mixtures by Liquid-Phase Isoelectric Focusing
Julie Hey, Anton Posch, Andrew Cohen, Ning Liu and Adrianna Harbers
241-256
Microscale Isoelectric Focusing in Solution
A Method for Comprehensive and Quantitative Proteome Analysis Using 1-D and 2-D DIGE Combined with MicroSol IEF
Prefractionation
Mee-Jung Han and David W Speicher
257-275
Prefractionation, Enrichment, Desalting and Depleting of Low Volume and Low Abundance Proteins and Peptides
Using the MF10
Valerie Wasinger, Linda Ly, Anna Fitzgerald and Brad Walsh
277-286
Sample Prefractionation in Granulated Sephadex IEF Gels
Angelika Görg, Carsten Lück and Walter Weiss
287-300
Free-Flow Electrophoresis System for Plasma Proteomic Applications
Robert Wildgruber, Jizu Yi, Mikkel Nissum, Christoph Eckerskorn and Gerhard Weber
301-313
Protein Fractionation by Preparative Electrophoresis
Michael Fountoulakis and Ploumisti Dimitraki
V / Enrichment Strategies for Organelles, Multiprotein Complexes, and Specific Protein Classes
315-315
Front matter
317-331
Isolation of Endocitic Organelles by Density Gradient Centrifugation
MarianaEÇaGuimarães Araùjo, Lukas Alfons Hube and Taras Stasyk
333-348
Isolation of Highly Pure Rat Liver Mitochondria with the Aid of Zone-Electrophoresis in a Free Flow Device (ZE-FFE)
Hans Zischka, Josef Lichtmannegger, Nora Jägemann, Luise Jennen and Daniela Hamöller, et al.
349-364
Isolation of Proteins and Protein Complexes by Immunoprecipitation
Barbara Kaboord and Maria Perr
365-372
Isolation of Phosphoproteins
Lawrence G. Puente and Lynn A. Megeney
373-396
Glycoprotein Enrichment Through Lectin Affinity Techniques
Yehia Mechref, Milan Madera and Milos V Novotny
397-401
Isolation of Bacterial Cell Membranes Proteins Using Carbonate Extraction
Mark P Molloy
403-412
Enrichment of Membrane Proteins by Partitioning in Detergent/Polymer Aqueous Two-Phase Systems
Henrik Everberg, Niklas Gustavasson and Folke Tjerned
413-422
The Isolation of Detergent-Resistant Lipid Rafts for Two-Dimensional Electrophoresis
Ki-Bum Kim, Jae-Seon Lee and Young-Gyu Ko
423-431
Isolation of Membrane Protein Complexes by Blue Native Electrophoresis
Veronika Reisinger and Lutz A. Eichacker
433-448
Tissue Microdissection
Heidi S. Erickson, John W. Gillespie and Michael R. Emmert-Buck
449-459
Back matter