• i-xxi

  Front matter

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• Part I / Detection and Quantification of HCV

• 1-1

  Front matter

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• 3-14

  Detection and Quantification of Serum or Plasma HCV RNA: Mini Review of Commercially Available Assays

  Helene Le Guillou-Guillemette and Francoise Lunel-Fabiani

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• 15-23

  Simultaneous Detection of Anti-HCV Antibody and HCV Core Antigen

  Filippo Ansaldi and Giancarlo Icardi

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• 25-30

  Immunohistochemical Detection of HCV Proteins in Liver Tissue

  Oivier Galy, Isabelle Vincent, Philippe Chevallier, Nicole Lyandrat and Isabelle Chemin

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• Part II / HCV Nomenclature, Genotyping, and Quasispecies

• 31-31

  Front matter

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• 33-53

  Nomenclature and Numbering of the Hepatitis C Virus

  Carla Kuiken and Peter Simmonds

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• 55-71

  A Real-Time Taqman® Method for Hepatitis C Virus Genotyping and Methods for Further Subtyping of Isolates

  Kathryn J. Rolfe, Tim G. Wreghitt, Graeme J. M. Alexander and Martin D. Curran

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• 73-81

  Characterization of HCV Quasispecies in the Hypervariable Region 1 (HVR 1) by In Vitro Translation and Mass Spectrometry

  Carmen Yea, Melissa Ayers and Raymond Tellier

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• Part III / HCV Replication in Vitro

• 83-83

  Front matter

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• 85-94

  Purification and Crystallization of NS5A Domain I of Hepatitis C Virus

  Joseph Marcotrigiano and Timothy Tellinghuisen

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• 95-110

  NS5A Phosphorylation and Hyperphosphorylation

  Petra Neddermann

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• 111-124

  Preparation and Handling of Hepatitis C Viral Proteins NS3 and NS5B for Structural Studies

  Stefania Di Marco and Andrea Carfí

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• 125-143

  Structural and Functional Analysis of the HCV p7 Protein

  Nathalie Saint, Roland Montserret, Christophe Chipot and François Penin

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• 145-163

  HCV Replicons: Overview and Basic Protocols

  Volker Lohmann

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• 165-175

  Reverse Transcription PCR-Based Sequence Analysis of Hepatitis C Virus Replicon RNA

  Timothy L. Tellinghuisen and Brett D. Lindenbach

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• 177-184

  Studying HCV RNA Synthesis In Vitro with Replication Complexes

  Wengang Yang and Mingjun Huang

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• 185-193

  Proteomics Study of the Hepatitis C Virus Replication Complex

  Kyungsoo Chang, Tianyi Wang and Guangxiang Luo

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• 195-209

  Investigation of the Hepatitis C Virus Replication Complex

  Volker Brass, Rainer Gosert and Darius Moradpour

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• 211-226

  Regulation of Interferon Regulatory Factor 3-Dependent Innate Immunity by the HCV NS3/4A Protease

  Kui Li

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• 227-242

  Selection and Characterization of Drug-Resistant HCV Replicons In Vitro with a Flow Cytometry-Based Assay

  Jason M. Robotham, Heather B. Nelson and Hengli Tang

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• 243-250

  High-Throughput Screening of HCV RNA Replication Inhibitors by Means of a Reporter Replicon System

  Weidong Hao and Rohit Duggal

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• 251-262

  Inhibition of HCV Replication by Small Interfering RNA

  Ratna B. Ray and Tatsuo Kanda

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• Part IV / HCV Entry and Infection

• 263-263

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• 265-277

  Cellular Receptors and HCV Entry

  Mike Flint and Donna M. Tscherne

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• 279-293

  Studying HCV Cell Entry with HCV Pseudoparticles (HCVpp)

  Birke Bartosch and François-Loïc Cosset

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• 295-304

  Screening of Small-Molecule Compounds as Inhibitors of HCV Entry

  Jian-Ping Yang, Demin Zhou and Flossie Wong-Staal

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• 305-327

  Isolation of JFH-1 Strain and Development of an HCV Infection System

  Takaji Wakita

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• 329-336

  Measuring HCV Infectivity Produced in Cell Culture and In Vivo

  Brett D. Lindenbach

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• 337-346

  Genotype 1a HCV (H77S) Infection System

  MinKyung Yi and Stanley M. Lemon

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• 347-359

  Full-Length Infectious HCV Chimeras

  Thomas Pietschmann

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• 361-372

  Adaptation of the Hepatitis C Virus to Cell Culture

  Artur Kaul, Ilka Wörz and Ralf Bartenschlager

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• 373-382

  Primary Human Hepatocyte Culture for HCV Study

  Haizhen Zhu, John Elyar, Robin Foss, Alan Hemming and Eric Hall, et al.

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• 383-399

  In Vivo Study of HCV in Mice with Chimeric Human Livers

  Norman M. Kneteman and Christian Toso

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• Part V / Immunity and Modeling

• 401-401

  Front matter

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• 403-413

  Determination of HCV-Specific T-Cell Activity

  Joo Chun Yoon and Barbara Rehermann

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• 415-426

  Analysis of HCV-Specific T Cells by Flow Cytometry

  Masaaki Shiina and Barbara Rehermann

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• 427-438

  Detection of Neutralizing Antibodies with HCV Pseudoparticles (HCVpp)

  Marlène Dreux and François-Loïc Cosset

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• 439-453

  Mathematical Modeling of HCV Infection and Treatment

  Harel Dahari, Emi Shudo, Ruy M. Ribeiro and Alan S. Perelson

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• 455-459

  Back matter

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