The main goal this paper is to describe a software simulating spatio-temporal Dengue epidemic spread based on the utilization of a generalized probabilistic cellular automata computational analysis as the dynamic model of spatial epidemiology. This epidemic spatial model permits to reproduce explicitly the interaction of two types of transmission mechanisms in terms of global and local variables, which in turn can be adjusted to simulate respectively the populational mobility and geographical neighborhood contacts. The resulting virtual laboratory was designed to run spatio-temporal simulation of the Dengue disease spreading based on local and global interactions among two distinct populations (humans and mosquitoes).