Bulletin of Experimental Biology and Medicine
Volume 145, Number 4, 504-510, DOI: 10.1007/s10517-008-0129-z

Translated from Kletochnye Tekhnologii v Biologii i Meditsine (Cell Technologies in Biology and Medicine)

Vital dye Dil and fluorescent magnetic microparticles do not affect the phenotype of mesenchymal stem cells from human amnion and their differentiation capacity

I. B. Cheglakov, A. N. Rytenkov and K. N. Yarygin

From the issue entitled "This issue is a translation of Byulleten’ Eksperimental’noi Biologii i Meditsiny (Bulletin of Experimental Biology and Medicine) and Kletochnye Tekhnologii v Biologii i Meditsine (Cell Technologies in Biology and Medicine)"

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Abstract

We present a method of labeling of mesenchymal stem cells from human amnion with a fluorescent dye Dil and microspheres (Bangs Laboratories). The possibility of administration of loaded cell culture was verified and comparative analysis of the phenotype of mesenchymal stem cells by the expression of fibronectin, nestin, CD13, CD29, CD34, CD44, CD54, CD90, CD105, CD106, HLA-ABC, HLA-DR, and PCNA was carried out. The labeled cells retained osteogenic differentiation capacity. The results suggest that fluorescent dye Dil and microspheres from Bangs Laboratories can be used for monitoring of mesenchymal stem cells from human amnion in in vivo experiments.

Key Words  mesenchymal stem cells - placenta - microparticles - phenotype - differentiation

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Translated from Kletochnye Tehnologii v Biologii i Meditsine, No. 2, pp. 73–79, April, 2008

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