Purpose
To assess the reproducibility of the new spectral domain Cirrus high-definition optical coherence tomography (HD-OCT; Carl
Zeiss Meditec, Dublin, CA, USA) for analysis of peripapillary retinal nerve fiber layer (RNFL) thickness in healthy eyes.
Methods
Thirty healthy Korean volunteers were enrolled. Three optic disc cube 200 × 200 Cirrus HD-OCT scans were taken on the same
day in discontinuous sessions by the same operator without using the repeat scan function. The reproducibility of the calculated
RNFL thickness and probability code were determined by the intraclass correlation coefficient (ICC), coefficient of variation
(CV), test-retest variability, and Fleiss’ generalized kappa (κ).
Results
Thirty-six eyes were analyzed. For average RNFL thickness, the ICC was 0.970, CV was 2.38%, and test-retest variability was
4.5 μm. For all quadrants except the nasal, ICCs were 0.972 or higher and CVs were 4.26% or less. Overall test-retest variability
ranged from 5.8 to 8.1 μm. The κ value of probability codes for average RNFL thickness was 0.690. The κ values of quadrants
and clock-hour sectors were lower in the nasal areas than in other areas.
Conclusions
The reproducibility of Cirrus HD-OCT to analyze peripapillary RNFL thickness in healthy eyes was excellent compared with the
previous reports for time domain Stratus OCT. For the calculated RNFL thickness and probability code, variability was relatively
higher in the nasal area, and more careful analyses are needed.
Keywords optical coherence tomography - reproducibility - retinal nerve fiber layer thickness
A shorter version of this paper was presented at the 2009 Annual Meeting of Association for Research in Vision and Ophthalmology,
3–7 May 2009, Fort Lauderdale, Florida