Volume 53, Number 1, 21-26, DOI: 10.1007/s00125-009-1556-8Open Access

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European Association for the Study of Diabetes

Pancreatic duct replication is increased with obesity and type 2 diabetes in humans

A. E. Butler, R. Galasso, A. Matveyenko, R. A. Rizza, S. Dry and P. C. Butler

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Abstract

Aims/hypothesis  

In a high-fat-fed rat model of type 2 diabetes we noted increased exocrine duct replication. This is a predisposing factor for pancreatitis and pancreatic cancer, both of which are more common in type 2 diabetes. The aim of the study reported here was to establish if obesity and/or type 2 diabetes are associated with increased pancreatic ductal replication in humans.

Methods  

We obtained pancreas at autopsy from 45 humans, divided into four groups: lean (BMI <25 kg/m2); obese (BMI >27 kg/m2); non-diabetic; and with type 2 diabetes. Pancreases were evaluated after immunostaining for the duct cell marker cytokeratin and Ki67 for replication.

Results  

We show for the first time that both obesity and type 2 diabetes in humans are associated with increased pancreatic ductal replication. Specifically, we report that (1) replication of pancreatic duct cells is increased tenfold by obesity, and (2) lean subjects with type 2 diabetes demonstrate a fourfold increase in replication of pancreatic duct cells compared with their lean non-diabetic controls.

Conclusions/interpretation  

Pancreatic duct cell replication is increased in humans in response to both obesity and type 2 diabetes, potentially providing a mechanism for the increased risk of pancreatitis and pancreatic cancer in those with obesity and/or type 2 diabetes.

Keywords  DPP-IV inhibitor - GLP-1 - Obesity - Pancreatic cancer - Pancreatic ductal - Pancreatitis

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