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Development of SCAR markers linked to three disease resistances based on AFLP within
Nicotiana tabacum
L
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Original Paper
Development of SCAR markers linked to three disease resistances based on AFLP within Nicotiana tabacum L
E. Julio1, J-L. Verrier1 and F. Dorlhac de Borne1 
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Altadis, Institut du Tabac, Domaine de la Tour, LBCM, 24100 Bergerac, France |
Received: 15 December 2004 Accepted: 29 September 2005 Published online: 11 November 2005
Communicated by A. Charcosset
Abstract Amplified fragment length polymorphism (AFLP) was conducted on a set of 92 Nicotiana tabacum L. accessions from diverse types (flue-cured, dark air-cured, burley, oriental, and cigar wrapper) and breeding origins to
identify markers associated with disease resistances. Eleven primer combinations were required to identify 33 polymorphic
fragments. This allowed the identification of 92% of these accessions, and yielded sufficient information for building a neighbor
joining tree. Clusters of accessions with common traits or breeding origins were observed. An important part of this polymorphism
could be related to interspecific introgressions from other Nicotiana species, performed during the breeding history of N. tabacum to confer resistance to pathogens. Seven fragments were associated with three different resistances: two for the blue-mold
( Peronospora tabacina Adam) resistance derived from Nicotiana debneyi Domin, two for the Va gene (Potato Virus Y susceptibility), and three for the black root rot ( Chalara elegans) resistance of N. debneyi origin. Some of these markers were converted into sequence characterized amplified region markers, and validated on recombinant
inbred lines or doubled-haploid lines.
Keywords Nicotiana tabacum - AFLP - Blue mold - PVYN
- Black root rot - SCAR markers
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