A method for the quantitative determination of L-alanine in triglycine sulphate crystals, in the presence of excess amounts of glycine, is described. L-alanine is determined after derivatization with 4-N,N-dimethyl-aminoazobenzene-4
-sulfonyl chloride (DABS-Cl) and in the presence of DABS-valine used as the internal standard. The results obtained indicate that the method permits the determination of alanine in triglycine sulphate crystals at concentrations of about 1%.
Key Words Column liquid chromatography - Quantitative determination of L-alanine - DABS-Cl - Tryglycine sulphate