Volume 14, Number 9, 501-506, DOI: 10.1007/BF02265628

On the use of the separation number as a criterion for the evaluation of gas chromatography capillary columns in isothermal conditions

J. Krupcik, J. Garaj, G. Guiochon and J. M. Schmitter

View Related Documents

Abstract

Although widely used to compare the performances of capillary columns the separation number is a poor index of the quality of a column. It is shown that it is a rubber ruler whose length is a function of temperature: the separation number of a column is a function not only of the effective plate number, which itself varies largely with the retention as measured by the capacity factor kprime, but also of the relative retention of then-alkanes used for the measurement. This relative retention is strongly dependent upon the temperature for a given stationary phase. Unless the temperature at which the separation number is measured be normalized as well as the kprime range in which the second alkane of the pair used is eluted, the separation numbers are meaningless for the appreciation of the performance of a column or comparison of different columns. Using alkanes eluted in the same kprime range to measure the separation number of a column we obtained a value almost twice as large at 80°C as at 180°C. In spite of their imperfections the plate number and the coating efficiency are easier to use and less misleading.

Key Words  Capillary gas chromatography - Separation number - Isothermal conditions

Fulltext Preview

Image of the first page of the fulltext document