In order to optimize tissue culture conditions for bermudagrass, an important warm-season turfgrass species, tissue culture
responses of young inflorescences of a hybrid bermudagrass cultivar `Tifgreen' (
Cynodon dactylon×
Cynodon transvaalensis) and a common bermudagrass cultivar `Savannah' (
Cynodon dactylon) were investigated. When cultured on Murashige and Skoog medium with 4.52 to 13.57 μM (1–3 mg l
-1) 2,4-D, young inflorescence segments yielded non-embryogenic calli which were unorganized and had loosely associated, long
tubular cells on the surface. However, inclusion of 6-benzyladenine (BA) in callus induction medium at a level of 0.044 μM
(0.01 mg l
-1) induced formation of a compact, nodular embryogenic structure on approximately 20% of the calli. Calli with such a compact
embryogenic structure were highly regenerable. When young inflorescences smaller than 0.75 cm were cultured, the embryogenic
structure yielded green plantlets with regeneration rates of 79.5% and 83.3%, respectively for the two cultivars. All 96 plants
regenerated from calli induced in the BA-containing medium were green and morphologically normal. The embryogenic nature of
the compact structure was confirmed by scanning electron microscopy.
Cynodon dactylon
-
Cynodon dactylon×Cynodon transvaalensis
- cytokinin - MS medium - plant tissue culture
This revised version was published online in June 2006 with corrections to the Cover Date.