Augmentin^® was used as an alternative antibiotic for suppressing Agrobacterium tumefaciens during tomato (Lycopersicon esculentum) transformation. The efficiency of Augmentin compared with Timentin^® to suppress the growth of Agrobacterium was at concentrations of 300 and 100 mg l^–1, respectively. In addition, the high concentration up to 500 mg l^–1 of both antibiotics showed no significant toxicity to shoot regeneration. The overgrowth of Agrobacterium was observed on tomato shoots regenerated on medium containing cefotaxime even at high concentration up to 500 mg l^–1. Moreover, shoot regeneration from medium containing cefotaxime was lower than the one from medium containing Timentin and Augmentin. However, we found that Timentin was more stable than Augmentin when stored as a mixed stock solution and kept at –20°C freezer. In order to increase the efficiency of Augmentin, we recommended the use of Augmentin at a concentration of 300 mg l^–1 with fresh preparation prior to use. Therefore, Augmentin can be used as another antibiotic for suppression of bacterial growth with comparative efficiency as Timetin for tomato transformation.