Aims/hypothesis
Circulating progenitor cells participate in cardiovascular homeostasis. Depletion of the pool of endothelial progenitor cells
(EPCs) is associated with increased cardiovascular risk. Furthermore, EPCs are reduced in the presence of classical risk factors
for atherosclerotic disease, including diabetes mellitus. This study was designed to evaluate progenitor cell levels in volunteers
with different degrees of glucose tolerance.
Methods
Cardiovascular parameters and the levels of circulating CD34+ and CD34+ kinase insert domain receptor (KDR)+ cells were determined in 219 middle-aged individuals with no pre-diagnosed alterations in carbohydrate metabolism. Glucose
tolerance was determined by fasting and 2 h post-challenge glucose levels, with IFG and IGT considered as pre-diabetic states.
Results
CD34+ and CD34+KDR+ cells were significantly reduced in individuals who were found to have diabetes mellitus, and were negatively correlated
with both fasting and post-challenge glucose in the whole population. While only CD34+ cells, but not CD34+KDR+ cells, were significantly reduced in pre-diabetic individuals, post-challenge glucose was an independent determinant of the
levels of both CD34+ and CD34+KDR+ cells.
Conclusions/interpretation
Glucose tolerance was negatively associated with progenitor cell levels in middle-aged healthy individuals. Depletion of endothelial
progenitors with increasing fasting and post-meal glucose may be one cause of the high incidence of cardiovascular damage
in individuals with pre-diabetes.
Keywords Atherosclerosis - Cardiovascular risk - Diabetes - Endothelium - Glucose tolerance - Pre-diabetes - Progenitor cells - Stem cells
G. P. Fadini and L. Pucci contributed equally to this study.