Calcium homeostasis and bone pathology were studied in weanling rats fed a low (70 ppm) magnesium diet for 2–21 days. The
rats developed significant, progressive hypercalcemia after 6 days on the diet. The increase in blood calcium was accompanied
by progressive hypoactivity of the parathyroid gland (PTG), as determined by histologic and morphometric analyses. Thus hyperactivity
of the PTG could not have been responsible for the hypercalcemia observed.
Histologic examination of femora and humeri from magnesium-deficient rats showed progressive subperiosteal hyperplasia, consisting
of undifferentiated osteoprogenitor cells and fibrous tissue, after 7 days of deficiency. The presence of unmineralized osteoid
tissue in the metaphyses indicated that mineralization was not proceeding normally. The alterations in differentiation of
osteoprogenitor cells, together with the failure of mineralization, resulted in significantly lower rates of bone formation
(as measured by fluorochrome labeling) in the magnesium-deficient rats. Basophilic cementing lines and inactive osteocytes
in the cortices of bones from magnesium-deficient rats indicated that bone resorption was also severely reduced in magnesium
deficiency. We postulate that bone magnesium depletion (66% by day 21) has a direct negative effect on osteoblastic and osteocytic
activity, and may explain, in part, the decreased responsiveness of bone to parathyroid hormone (PTH) that has been observed
in magnesium-deficient animals.
Key words Magnesium - Bone - Calcium - Parathyroid gland