Focus on Biotechnology, 2002, Volume 4, Part VII, 339-349, DOI: 10.1007/0-306-46889-1_22

High-Speed Pectic Enzyme Fractionation by Immobilised Metal Ion Affinity Membranes

Silvia Camperi, Mariano Grasselli and Osvaldo Cascone

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Abstract

Immobilised metal ion affinity polysulphone hollow-fibre membranes with a high capacity for protein adsorption were prepared and their application for commercial pectic enzyme fractionation was studied. The pass-through fraction containing pectin lyase (PL) is useful for fruit-juice clarification without methanol production on account of pectin-esterase (PE) being retained by the IDA-Cu2+ membrane.

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