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Characterization, cloning and sequencing of a thermostable endo-(1,3–1,4) β-glucanase-encoding gene from an alkalophilic Bacillus brevis
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Applied Genetics and RegulationCharacterization, cloning and sequencing of a thermostable endo-(1,3–1,4)  -glucanase-encoding gene from an alkalophilic Bacillus brevis
Maureen E. Louw1, Sharon J. Reid1 and T. G. Watson2 | (1) | Biotechnology Programme, Division of Food Science and Technology, CSIR, P. O. Box 395, 0001 Pretoria, Republic of South Africa |
| (2) | Department of Microbiology, University of Cape Town, Private Bag, Rondebosch 7700, Cape Town, Republic of South Africa |
Received: 21 April 1992 Accepted: 3 August 1992 Abstract A Bacillus brevis gene coding for an endo-(1,3–1,4)-  -glucanase was cloned in Escherichia coli and sequenced. The open reading frame contains a sequence of 759 nucleotides encoding a polypeptide of 252 amino acid residues. The amino acid sequence of the -glucanase gene showed only a 50% similarity to previously published data for Bacillus endo-(1,3–1,4)- -glucanases. The optimum temperature and pH for enzyme activity were 65–70°C and 8–10, respectively. When held at 75°C for 1 h, 75% residual activity was measured. The molecular mass was estimated to be about 29 kDa on sodium dodecyl sulphate (SDS)-polyacrylamide gel electrophoresis and the enzyme was found to be resistant to SDS.
Correspondence to: T. G. Watson
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