Amyloid-containing (A+) islets are characteristic for type-2 diabetes (T2D), but their abundance seems variable among patients. It is unclear whether the distribution of A+ islets follows a certain pattern or occurs randomly throughout the pancreatic organ. We investigated the topography of A+ islets in eight pancreata of T2D patients and eight sex- and age-matched non-diabetic subjects. Transversal sections of head, body and tail segments were stained with synaptophysin combined with Congo red to map/quantify islet tissue and amyloid. In the eight T2D pancreata, the overall percentage of A+ islets varied from 4% to 85%. Further analysis in body and tail indicated that peripheral regions exhibited higher percentages of A+ islets than central regions (averages of, respectively, 30% and 17%, P<0.05). non-diabetic="" control="" pancreata="" also="" exhibited="" a+="" islets,="" albeit="" at="" a="" 25-fold="" lower="" frequency;="" a="" tendency="" towards="" higher="" percentage="" of="" a+="" islets="" in="" peripheral="" versus="" central="" regions="" was="" also="" observed.="" the="" higher="" percentage="" a+="" islets="" in="" peripheral="" regions="" was="" associated="" with="" a="" higher="" density="" and="" relative="" islet="" over="" exocrine="" surface="" area.="" these="" observations="" on="" heterogeneity="" in="" abundance="" and="" distribution="" of="" a+="" islets="" need="" consideration="" when="" sampling="" tissue="" for="" studies="" on="" human="" islet="" amyloidosis.="" the="" present="" methodology="" allows="" us="" to="" further="" investigate="" the="" susceptibility="" to="" amyloidosis="" of="" islets="" in="" peripheral="" regions="" of="" the="">