Volume 32, Number 2, 115-125, DOI: 10.1007/BF02049626

Permanent mounts of fungus cultures grown on media optimal for production of characteristic structures

E. M. O'Hern and M. G. Levine

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Abstract

1. Dermatophyte cultures in plastic flasks were compared on four different media for total growth, sporulation, and pigment production. Czapek's agar favored more rapid and more abundant sporulation in most cultures than Sabouraud's dextrose agar, potato dextrose agar, or wort agar.
2. A simple technique for embedding agar island cultures of fungi is described. These plastic-embedded cultures form permanent mounts useful for study of microscopic morphology.
3. A relatively durable slant culture in a similar plastic flask is suggested as a companion for study of gross morphologic features of the cultures.

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