Chromosome samples were prepared on a plastic coverslip covered with a polyester membrane and were subjected to the C-banding treatment. The C-band pattern was obtained after Giemsa staining. The C-band positive regions of the
Crepis chromosomes were identified, dissected out by irradiation with a micro-laser beam and recovered in Eppendorf tubes.
Key words Laser dissection - Micromanipulation -
Crepis capillaris
- C-banding - Quantitative ideogram
Communicated by F. Mechelke