The expression of the adenylate kinase isozymes and of phosphopyruvate hydratase was studied in man-mouse and man-hamster hybrid clones. Concordant segregation of the loci coding for AK-2 and PPH was observed in 54 of 55 primary hybrid clones, and these loci were demonstrated to be synthetic with the loci specifying PGM-1 and PGD. The pattern of expression of the four enzymes in discordant clones suggests the gene order 1pter-(PGD,PPH)-AK-2-PGM-1-centromere. In addition, AK-1 was found to be expressed independently of AK-2.
Key words adenylate kinase isozymes - phosphopyruvate hydratase - 6-phosphogluconate dehydrogenase - phosphoglucomutase-1 - chromosome 1 - man-rodent somatic cell hybrids
This work was supported by the NIH Grants 5 PO1 HB 06276-04, HD 04807-06, and HD 06285-04.