OBJECTIVE To examine the antitumor activities of fresh apple extracts.
METHODS Fuji apple extracts were tested for their anti-LS-174T-proliferative activities, for their effect on expression of PCNA,
and ability to induce apoptosis in a LS-174T cell line.
RESULTS Apple extracts inhibited LS-174T cellular proliferation in a concentration and time dependent manner. The apple extracts
equivalent to a concentration of 50 mg/ml inhibited the proliferation of the LS-174T cells by 34.5±1.2% after 48 h and 47.5±1.8%
after 72 h respectively. Apple extracts inhibited PCNA expression and induced apoptosis of the LS-174T cells at concentrations
above 12.5mg/ml.
CONCLUSION Apple extracts can inhibit PCNA expression and induction of apoptosis in LS-174T cells which may contribute to their inhibitiory
effect on cellular proliferation.
Keywords apple - LS-174T cell lines - phytochemicals - PCNA - apoptosis
This work was supported by the Overseas Chinese Scholars Fund of Educational Ministry, China (No. [2003] 406).