The lack of knowledge of current public attitudes towards basic research into induced pluripotent stem cells (iPSCs) is a serious problem when considering appropriate ways of governance regarding research and its clinical applications. We therefore conducted an internet-based survey to determine public opinion regarding the research and development of iPSCs and regenerative medicine (RM). A total of 14,908 valid responses were collected, which revealed that the Japanese public were familiar with the terms iPSCs and RM, and many of them had received information about iPSCs and RM through the television and newspapers. They also generally accepted the need for extra funding for research into iPSCs, but also decided to adopt a “wait and see” approach and thought that research and development of iPSCs and RM should be conducted under proper governance in accordance with an international regulatory framework. It will be necessary to discuss an internationally consistent regulatory system and effective mechanisms for information flow.