Infectivity of non-occluded particles of nuclear polyhedrosis virus infecting
Galleria mellonella L. was determined with bioassay technique
in vivo and with plaque assay method
in vitro using SCLd135 (Quiot) established cell culture. A plaque-forming virus unit corresponds to about 50 ID
50 for
Galleria larvae injected into hemocoel for non-cloned and 0.1–1.0 ID
50 for a virus isolate cloned from an infective plaque.