By means of histoenzymological techniques, the formation and degradation of the following enzymes have been related to phases in the development of the fish ovary.
| 1. |
3\gb-Hydroxysteroid dehydrogenase, in follicular or thecal cells of maturing oocytes, disappears in big follicles before spawning.
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| 2. |
Glucose-6-phosphate dehydrogenase, in immature oocytes, was localized in the cytoplasm, and especially in the yolk nucleus.
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| 3. |
\ga-Glycerophosphate dehydrogenase was visualized in lamella connective tissue in the stroma, as well as in the yolk nucleus of young oocytes.
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| 4. |
Succinate dehydrogenase was particularly apparent in the cytoplasmic yolk nucleus, presumably rich in mitochondria.
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| 5. |
Alkaline phosphatase was abundant in thecal cells of maturing follicles during yolk synthesis.
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| 6. |
Acid phosphatase was present in the peripheral layer of the cytoplasm of resorbing oocytes.
The enzymatic constitution of the fish oocyte showed two main synthetic phases:
| (a) |
The first phase, characterized by the richness of the immature oocytes in NAD and NADP-linked dehydrogen platelets.
| | (b) |
The second phase, in mature oocytes, involves storage of nutrient substances and steroid shormone formation by the two oocyte layers of follicular and thecal cells.
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