Environmental changes of the last 9,300 years were reconstructed by geochemical and pollen analyses of a 14-m-long, laminated sediment core from Lago Aleixo, south-eastern Brazil. Fossil pollen assemblages indicate open savannah vegetation (campo cerrado) and gallery forests until approximately 6,900 cal. BP. During that time, siderite laminae were deposited under anoxic conditions at the lake bottom. Then, increased rainfall and a shorter annual dry period allowed gallery forests and semi-deciduous forests to expand, leading to more closed cerrado vegetation. High-intensity rainfall events during this period are recorded as peaks in K and Ti concentrations. The sediment facies during this period consists of alternating layers of diatoms and minerogenic matter. C/N ratios imply that algae and perhaps soils, too, were the main contributors to sediment organic matter. Biogenic silica and δ¹³C_org variations indicate increasing primary productivity, which was related to higher nutrient flux from intensified leaching of soils, as shown by rising K/Al ratios. Around 800 cal. BP, a closed, semi-deciduous forest developed under present-day climate conditions. Slope stabilization diminished erosion processes in the catchment and caused reduced input of minerogenic matter into the basin. Human impact is evident in the topmost homogeneous sediments, as removal of the stabilizing forest cover amplified soil erosion. The continuous trend to more humid conditions during the Holocene probably reflects increased influence of the Amazon Basin as a moisture source. We conclude that the Lago Aleixo sediment archive was a sensitive recorder of environmental dynamics in tropical South America, which were mainly controlled by changes in precipitation patterns.