The expression characteristic of two granule-associated genes,
phaP and
phaR, in a recombinant
E. coli harboring the
phbCAB operon was investigated. Polybetamydroxybutyrate (PHB) accumulation increased up from 16% to 57% (w/w) after transformation of the granule-associated genes due to the stabilization of PHB granules rather than by a direct effect on PHB biosynthetic enzymes. The morphology of PHB granules also varied depending on the transformed
phaP and
phaR genes.
granule-associated genes -
phaP
-
phaR
- poly-
-hydroxybutyrate -
Ralstonia eutropha
- recombinant E. coli