The origin of lentil from the taxon Lens culinaris subsp. orientalis has been proved by morphological evidence and breeding experiments. This wild form exhibits variation in many characters and is distributed over a vast area from the Middle-East to central Asia. Characters that are polymorphic in the wild progenitor but monomorphic in the cultigen can be utilized for better identification of the genetic stock which gave rise to the domesticated lentil. Three characters of that kind have been identified in lentil: chromosomal architecture, crossability potential and restriction pattern of chloroplast DNA. Nearly all accessions of the cultivated lentil tested to these three characters have been found monomorphic, but considerable polymorphism exists in the wild accessions. Three subsp. orientalis accessions have been shown to share the above characters with the cultigen and hence can be regarded as members of the genetic stock from which lentil was domesticated. These three accessions originated from eastern Turkey and northern Syria.