SomeArxula adeninivorans strains selected from wood hydrolysates in Siberia, from soil in South Africa and from maize silage and soil in The Netherlands were compared. DNA-fingerprinting, pulse field gel electrophoresis as well as analysis of secretory proteins have been chosen to describe the similarities among the strains. Combination of the three methods allowed identification of each strain. Strains from the same origin show extensive similarities. The results of the DNA-fingerprints indicate that the strain isolated in Siberia belongs to the group of strains originated from South Africa. However, it differed in the molecular weight of the third chromosome and in the pattern of secretory proteins from the South African isolates.