Analysis and detection of DNA variation is important in any field of biology. Hence, numerous methods have been developed
to analyze DNA. A simple yet effective way of analyzing DNA is by denaturant capillary electrophoresis (DCE). The method is
in theory applicable to 95% of the human genome. The method involves three steps; fragment design, PCR amplification and allele
separation. The allele separation can in principle be performed with any DNA capillary sequencing instrument.
Keywords DGGE (denaturant gradient gel electrophoresis) – CDCE (constant denaturant capillary electrophoresis) – CTCE (cycling temperature capillary electrophoresis) – PCR (polymerase chain reaction) – dsDNA — double strand DNA – ssDNA — single strand DNA