The circumscription of theIsertieae has been under debate for a long time and recently a phylogeny based on morphological data has been presented (Andersson 1996), contradicting the classification ofRobbrecht (1988, 1993). Our investigation of molecular data neither supports the phylogeny ofAndersson nor the classification ofRobbrecht, but instead indicates totally new relationships ofIsertieae, Mussaendeae, andSabiceeae. TheIsertieae are a bigeneric tribe of subfam.Cinchonoideae, whileMussaendeae andSabiceeae are two separate tribes of subfam.Ixoroideae. We have also referred a species from Socotra (Yemen) with disputed position to the tribeSabiceeae and we place it in a new genus,Tamridaea, with the single speciesT. capsulifera comb. nov. NewrbcL sequences of 20 taxa are presented and analysed, fromGentianaceae:Gentianella; fromLoganiaceae:Spigelia; and fromRubiaceae:Amphidasya, Aoranthe, Chomelia, Coussarea, Gonzalagunia, Heinsia, Hippotis, Isertia (three taxa),Mussaenda, Pseudomussaenda, Pseudosabicea, Rondeletia, Sabicea, Schradera, Tamridaea, andVirectaria.