Retinal projections were studied in the tree shrew, Tupaia glis, by means of thaw-mount autoradiography. In this technique, unfixed and unembedded frozen sections are directly mounted on photographic emulsion coated slides. Loss of radiolabeled material through tissue processing is avoided, probably resulting in increased discriminatory sensitivity. Together with multiple injections of precursor cocktail it is possible to demonstrate at the light microscopic level (1) fibers in passage and axon terminals simultaneously, and (2) preferentially labeled axon terminals in the projection field as areas of greater grain density.