The disk electrophoresis of peptide fragments from porcine pepsin obtained by cleaving the pepsin with cyanogen bromide has
been performed. A method has been developed for extracting the colored peptide fractions after electrophoresis in polyacrylamide
gel. An amino-acid analysis has been performed of peptide fragments B1, B2, and B4 isolated by disk electrophoresis. The results
obtained have been compared with the amino-acid composition calculated from the structures of these peptides. The possibility
has been shown of using the method described during structural investigations of both proteins and their low-molecular-weight
fragments.
The peptide preparations were supplied to us by I. Surova, V. Ostoslavskaya, L. Revina, I. Puracheva, É. Vakhitova, and G.
Muratova. The amino-acid analysis was performed by E. Timokhina, A. Balduev, G. Fedyukina, O. Khodova, and N. Ivashechkina.
All-Union Scientific-Research Institute of the Genetics and Breeding of Industrial Microorganisms. Translated from Khimiya
Prirodnykh Soedinenii, No. 2, pp. 219–222, March–April, 1975.