Production of β-cyclodextrin (CD) with high-dextrose equivalent (DE) starch hydrolysates by simultaneous fermentation and
cyclization (SFC) gives higher yields than using only the enzyme CGTase, because fermentation eliminates glucose and maltose
that inhibit CD production, while at the same time, produces ethanol that increases yield. A 10% (w/v) solution of cassava
starch, liquefied with α-amylase, was incubated with CGTase using: only the enzyme, added ethanol (from 1 to 5%), and added
yeast,
S. cerevisiae (12% w/v), plus nutrients, the latter being the SFC process. Reaction conditions were: 38αC, pH 6.0, DE from 2 to 25, and
3.3 mL of CGTase/L. The yield of β-CD has decreased with an increase in DE, and maximum reaction yields were found for DE
equal to 3.54, reaching 5.6, 14.7, and 11.5 mM β-CD, respectively. For an increase of DE, of approx 6 times (from 3.54 to
23.79), β-CD yield decreased 6 times for the first, and second reaction media with 3% (v/v) ethanol, and only approx 3 times
for SFC (from 11.5 to 3.73 m
M), showing that this process is less sensitive to variations in the DE
Index Entries Cyclodextrin - CGTase - fermentation - cassava starch