Volume 91, Numbers 6-7, 1092-1094, DOI: 10.1007/BF00223924

Identification of a RAPD marker linked to the pendula gene in Norway spruce (Picea abies (L.) Karst. f. pendula)

A. Lehner, M. A. Campbell, N. C. Wheeler, T. Pöykkö, J. Glössl, J. Kreike and D. B. Neale

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Abstract

The pendula phenotype of Norway spruce [Picea abies (L.) Karst f. pendula] is characterized by narrow crowns and strong apical dominance and is controlled by a single dominant gene (P). This defined genetic control presents one of the few opportunities to map a single gene controlling a morphological trait in a forest tree. We used random amplified polymorphic DNA (RAPD) markers and bulked segregant analysis to identify one locus OPH10_720, linked to the pendula gene. The estimated recombination frequency (r) between OPH10_720 and P was 0.046 (SE r =0.032). Mapping of the pendula gene is an important first step towards the ultimate identification and cloning of this gene.

Key words  Norway spruce - Pendula phenotype - Bulked segregant analysis - RAPD markers

Communicated by P. M. A. Tigerstedt

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