In order to determine the effect of substrate nitrogen (N) on ligninolytic activity,
Pleurotus ostreatus was grown in solid media containing either growth-limiting (1mM) or excess (10mM) NH
4Cl. After 25 days,
14C–CO
2 production from
14C-cornstover lignin in low-N medium was 3 times that in nitrogen (N)-rich medium. Supplementation of low-N medium with glucose (0.3%) further enhanced ligninolytic activity. Decolorization of an aromatic, polymeric dye, Poly R-481, in solid media was also greatest under N-limiting conditions.
Key words
Pleurotus ostreatus
- Poly R-481 - Cinnamic acid - Biodegradation - Secondary Metabolism - Nitrogen Catabolite Repression - White Rot - Cornstover - Bioconversion - Lignin