Volume 44, Number 3, 183-188, DOI: 10.1007/BF00048522

Shoot tips of wheat as an alternative source for regenerable embryogenic callus cultures

Kirsten Viertel and Dieter Hess

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Abstract

Shoot tips of Triticum aestivum L. cvs. Turbo and Nandu, both summer wheat varieties, were excised from 4 and 10 day-old seedlings, and used for induction of embryogenic callus. A modified L3 medium, supplemented with 10 mgrM 2,4-dichlorophenoxyacetic acid (2,4-d) for culture initiation, and 5 mgrM 2,4-d for subculturing, was optimal; 90% of 4 day-old lsquoTurborsquo seedlings formed embryogenic callus. Optimal plant regeneration was achieved from callus incubated on a modified MS medium without 2,4-d, but supplemented with 2.22 mgrM 6-benzylaminopurine and 0.27 mgrM naphthaleneacetic acid. Plantlets formed via embryogenesis from all embryogenic lsquoTurborsquo calli initiated from 4 day-old explants, with a mean number of 8 regenerants per explant. Regeneration occured via embryogenesis only. Results obtained using lsquoNandursquo were within the same range.

Key words  clonal propagation - somatic embryogenesis - shoot tip -  Triticum aestivum L.

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